Instruments

The LACA volunteers obtain data using several methods and instruments.  The primary instruments used by the LACA volunteers include the in-Situ SmarTROLL Multiparameter (MP) instrument, the Turner Designs CyanoFluor instrument, the MBio instrument and a Secchi Disk.  Each of these instruments and the data they provide are explained below.


In-Situ SMARTROLL Multiparameter Handheld Unit Water Quality Monitoring System

The in-Situ SmarTROLL Multiparameter (MP) handheld unit water quality monitoring system is an electronic data collection system used in the LACA program to measure air temperature, water temperature, pH, dissolved oxygen (DO), and specific conductance.  We take readings of these parameters at four depths (0.3, 1, 2, and 3 meters).  Air and water temperatures are measured and recorded to have a record of the conditions under which the other data are obtained. 


Tuner Designs CyanoFluor(TM) Instrument

The CyanoFluor handheld instrument is used by LACA to detect the presence of chlorophyll (CHL) and phycocyanin (PC) pigments in water samples.  The CyanoFluor instrument calculates and reports a PC to CHL ratio that estimates the relative abundance of cyanobacteria, algae that are known to cause Harmful Algal Blooms (HABs).  LACA monitors these PC to CHL ratios from May through October.  Data from these monitoring events will provide invaluable information to LACA and our water quality partners on changing environmental conditions that could favor cyanobacterial production, potentially leading to cyanobacterial blooms.  Our monitoring efforts using the CyanoFluor instrument are used to predict the onset of HABs so we can provide information to our membership and State agencies on the state of algal presence in the lake.  The Virginia Department of Environmental Quality (DEQ) and Virginia Department of Health (VDH) monitor the LACA data, along with data collected at regular intervals by DEQ, to ensure proper protocols are implemented to prevent or reduce harmful algal bloom effects.


MBio Diagnostics MQ Reader Instrument

The MBio MQ Reader is an automated, fluorescence-based microarray analysis system intended for the rapid quantitative measurement of analytes within a fluidic sample.  The instrument utilizes MBio’s proprietary LightDeck® technology to capture images of the fluorescence generated by the microarray.   The MQ Reader works with a single use, disposable, cartridge which contains a printed microarray.  A small volume of our collected water sample is introduced into the cartridge and the cartridge is then placed in the reader.  The water sample flows into a fluidic channel over the printed microarray and the MQ Reader analyzes the sample and provides a data output.   If toxins are present in the sample, the data output provides the measured quantities of toxin.  LACA reports all measured levels of toxins to DEQ and VDH as well as several of our academia partners.

The MBio MQ Reader is capable of measuring two of the primary toxins found in freshwater, microcystin and cylindrospermopsin.  These two toxins can have negative impacts on humans and animals when exposure to high levels occurs.  See the LACA Water Quality Data web page for current readings and maps of algal and toxin concentrations.


Secchi Disk

The Secchi disk is used to measure how far visible light can pass through the water.  This provides an indication of the effect suspended materials in the water have on water clarity.  This measurement provides us an indication of the turbidity of the water.  A disk with a black and white pattern is lowered into the lake by a measured line until the observer loses sight of it.  The disk is then raised until it reappears. The depth at which the disk vanishes and the depth at which it reappears are recorded.  The average of these two depths is the Secchi disk reading.

The Secchi disk data does not provide a direct indication of algal density in the water.  There are many other factors found both inside and outside the lake that affect how deep a person can see into the water – such as suspended sediments, weather and water surface conditions.  LACA utilizes the CyanoFluor instrument (see above) to more closely measure the amount of algae in the water.

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